People with cystic fibrosis, pancreatitis, or malabsorption may not make enough trypsin enzyme on their own, so they might ...

9| Digest the protein with trypsin while it is still in the gel, and electroelute the peptides. (i) Remove the gel slice to a clean surface, and allow it to partially dry. (ii)Add 5 μl of trypsin ...

Protein standards to test that trypsin digestion, peptide elution, chromatography and MS are in working order are essential. The assignment of spectra to peptides and proteins should also be ...

Here are some of the main proteases found in the body: In addition to pepsin, trypsin, and chymotrypsin, the body produces many other proteases to use for essential processes, such as digestion ...

Both instruments are in our open access sample prep facility. Before being analyzed by mass spec, most proteins must be broken into smaller fragments. This is accomplished by digestion with a protease ...